High-resolution 3D isotropic MR imaging of mouse flank tumours obtained in vivo with solenoid RF micro-coil.

TitleHigh-resolution 3D isotropic MR imaging of mouse flank tumours obtained in vivo with solenoid RF micro-coil.
Publication TypeJournal Article
Year of Publication2008
AuthorsHolmes WM, Maclellan S, Condon B, Dufès C, Evans TRJ, Uchegbu IF, Schätzlein AG
JournalPhys Med Biol
Volume53
Issue2
Pagination505-13
Date Published2008 Jan 21
ISSN0031-9155
KeywordsAbdominal Neoplasms, Animals, Anisotropy, Carcinoma, Squamous Cell, Female, Image Enhancement, Imaging, Three-Dimensional, Magnetic Resonance Imaging, Magnetics, Mice, Miniaturization, Reproducibility of Results, Sensitivity and Specificity
Abstract

The investigation of mouse flank tumours by magnetic resonance imaging (MRI) is limited by the achievable spatial resolution, which is generally limited by the critical problem of signal-to-noise ratio. Sensitivity was improved by using an optimized solenoid RF micro-coil, built into the animal cradle. This simple design did not require extensive RF engineering expertise to construct, yet allowed high-resolution 3D isotropic imaging at 60 x 60 x 60 microm(3) for a flank tumour in vivo, revealing the heterogeneous internal structure of the tumour. It also allowed dynamic contrast enhanced (DCE) experiments and angiography (MRA) to be performed at 100 x 100 x 100 microm(3) resolution. The DCE experiments provided an excellent example of the diffusive spreading of contrast agent into less vascularized tumour tissue. This work is the first step in using high-resolution 3D isotropic MR to study transport in mouse flank tumours.

DOI10.1088/0031-9155/53/2/015
Alternate JournalPhys Med Biol
PubMed ID18185002
Grant List / / Cancer Research UK / United Kingdom